Engineering cell-material interfaces for long-term expansion of human pluripotent stem cells.

TitleEngineering cell-material interfaces for long-term expansion of human pluripotent stem cells.
Publication TypeJournal Article
Year of Publication2013
AuthorsC-W Chang, Y Hwang, D Brafman, T Hagan, C Phung, and S Varghese
JournalBiomaterials
Volume34
Start Page912
Issue4
Pagination912 - 921
Date Published01/2013
Abstract

Cost-effective and scalable synthetic matrices that support long-term expansion of human pluripotent stem cells (hPSCs) have many applications, ranging from drug screening platforms to regenerative medicine. Here, we report the development of a hydrogel-based matrix containing synthetic heparin-mimicking moieties that supports the long-term expansion of hPSCs (≥20 passages) in a chemically defined medium. HPSCs expanded on this synthetic matrix maintained their characteristic morphology, colony forming ability, karyotypic stability, and differentiation potential. We also used the synthetic matrix as a platform to investigate the effects of various physicochemical properties of the extracellular environment on the adhesion, growth, and self-renewal of hPSCs. The observed cellular responses can be explained in terms of matrix interface-mediated binding of extracellular matrix proteins, growth factors, and other cell-secreted factors, which create an instructive microenvironment to support self-renewal of hPSCs. These synthetic matrices, which comprise of "off-the-shelf" components and are easy to synthesize, provide an ideal tool to elucidate the molecular mechanisms that control stem cell fate.

DOI10.1016/j.biomaterials.2012.10.020
Short TitleBiomaterials
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