Molecularly Tailored Interface for Long-Term Xenogeneic Cell Transplantation

TitleMolecularly Tailored Interface for Long-Term Xenogeneic Cell Transplantation
Publication TypeJournal Article
Year of Publication2022
AuthorsSK Madhurakka Perikamana, N Seale, J Hoque, JH Ryu, V Kumar, YV Shih, and S Varghese
JournalAdvanced Functional Materials
Volume32
Issue4
Pagination2108221 - 2108221
Date Published01/2022
Abstract

Encapsulation of therapeutic cells in a semipermeable device can mitigate the need for systemic immune suppression following cell transplantation by providing local immunoprotection while being permeable to nutrients, oxygen, and different cell-secreted biomolecules. However, fibrotic tissue deposition around the device has been shown to compromise the long-term function of the transplanted cells. Herein, a macroencapsulation device design that improves long-term survival and function of the transplanted cells is reported. The device is comprised of a semipermeable chitosan pouch with a tunable reservoir and molecularly engineered interface. The chitosan pouch interface decorated with 1,12-dodecanedioic acid (DDA), limits the cell adhesion and vigorous foreign body response while maintaining the barrier properties amenable to cell encapsulation. The device provides long-term protection to the encapsulated human primary hepatocytes in the subcutaneous space of immunocompetent mice. The device supports the encapsulated cells for up to 6 months as evident from cell viability and presence of human specific albumin in circulation. Solutions that integrate biomaterials and interfacial engineering such as the one described here may advance development of easy-to manufacture and retrievable devices for the transplantation of therapeutic cells in the absence of immunosuppression.

DOI10.1002/adfm.202108221
Short TitleAdvanced Functional Materials